Catalog Number - 68304-10P-2

Cogent RP C4 Butyl™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic to moderately hydrophobic compounds. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, it provides excellent reproducibility, reliable peak shape, and balanced retention characteristics. As a USP L26 equivalent phase, it is commonly used as an alternative for compendial methods and for applications where lower hydrophobic retention than C8 or C18 phases is preferred.

68304-10P-2 is an HPLC column packed with 3 µm RP C4 Butyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. This popular narrow-bore configuration combines improved chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 100 mm length provides greater peak capacity and resolving power than shorter 50 mm columns, while the 3 µm particles deliver sharper peaks and higher efficiency than comparable 5 µm packings. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of precise and reproducible low-flow operation.

This column is particularly well suited for hydrophobic and neutral compounds analyzed by reversed-phase HPLC. The C4 chemistry provides lower retention than C8 phases, making it advantageous when faster elution and shorter run times are desired. The 2.1 mm ID format is also a popular choice for LC-MS applications where lower solvent consumption and improved detector response are beneficial.

Key Benefits

• RP C4 Butyl™ stationary phase

• USP L26 equivalent chemistry

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Improved peak capacity

• Ideal for LC-MS methods

• Reduced solvent consumption

Typical Applications

• USP L26 methods

• Reversed-phase HPLC

• LC-MS methods

• Small molecule analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Impurity profiling

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC and LC-MS systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 100 mm length provides greater resolution and peak capacity than 50 mm columns

• Lower retention than comparable C8 stationary phases

• Well suited for hydrophobic and neutral compounds

• Commonly used as an alternative for USP L26 methods

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